Statistical method based solid state fermentative bioprocess development for the production of polygalacturonase by Rhizopus stolonifer | |
---|---|
( Volume 2 Issue 1,January 2016 ) OPEN ACCESS | |
Author(s): | |
Vijayshekhar.C.K, S. Manohar, J. Lalitha | |
Abstract: | |
Wheat bran is one of the major wastes from food processing industries, which poses considerable disposal problems and ultimately leads to environmental pollution. The main objective of the current research work was to determine the significant parameters on the production of industrially important polygalacturonase enzyme from wheat bran. Solid state culture conditions for polygalacturonase production by Rhizopus stolonifer from wheat bran were optimized by Taguchi’s L-18 orthogonal array experimental design methodology. Eight fungal metabolic influencing variables, viz. temperature, moisture, wheat bran, inoculum, pectin, NH4NO3, MgSO4, KH2PO4, and incubation time are used. The optimum conditions for predicted maximum polygalacturonase production were found to be pH 7, temperature 30 °C and fermentation period of 84 hrs. |
|
Paper Statistics: | Total View : 438 | Downloads : 429 | Page No: 34-38 | |
Cite this Article: | Click here to get all Styles of Citation using DOI of the article. |