Effects of solvent polarity and acidity on the extraction efficiency and fatty acid compositions of oil from Nile crocodile | |
---|---|
( Volume 1 Issue 1,July 2015 ) OPEN ACCESS | |
Author(s): | |
Yi Huang, Shengzhao Dong, Yun Liu | |
Abstract: | |
Background: Crocodile oil extracted from the fatty tissues of crocodiles is rich in monounsaturated and polyunsaturated fats, which have high economic and medical values. The present study describes and compares the effects of polarity and acid of extraction solvents on the extraction efficiency and fatty acid compositions of crocodile oil extracted by six solvents extraction systems, which are the combinations of three polarity levels (75% CH2Cl2, 50% CH2Cl2 and 25% CH2Cl2) and two acidity levels (non-acidified and acidified). Results: The oil extract was saponification with 0.5M KOH before re-suspected by n-hexane, and the fatty acid compositions of lipids was analyzed by gas chromatography-Mass Spectrometer (GC-MS). Analysis of variance (ANOVA) shows that solvent polarity not acidity of six extraction solvent systems has a significant effect on the oil yield. Different solvent systems could extract an obvious different type and amount of fatty acids compositions, which consisted of long carbon chains fatty acids. Among the six solvent systems examined, 50% CH2Cl2 + 46.7% methanol without acid was the best for extraction of oils from crocodile fat. DPPH antioxidation test indicated that crocodile oil extracted by solvent systems of 50% CH2Cl2 + 46.7% methanol without acid showed higher antioxidation activity (77.5%) than oil (56.0%) extracted by petroleum ether. Conclusions: The findings in this work are very helpful to screen the extraction solvent systems to extract the oil from crocodile organism. |
|
Paper Statistics: | Total View : 501 | Downloads : 492 | Page No: 1-6 | |
Cite this Article: | Click here to get all Styles of Citation using DOI of the article. |